New technique for rapid purification, entrapment, and recovery of enterotoxin A from a liquid chamber by polyacrylamide gel electrophoresis.
نویسندگان
چکیده
Electrophoreis of enterotoxin into a middle liquid section of a polyacrylamide gel column enhances recovery for serological assay.
منابع مشابه
Improved method for purification of enterotoxin from Clostridium perfringens type A.
The purification procedure of Clostridium perfringens type A enterotoxin has been improved. The cell sonic extract was precipitated twice with ammonium sulfate, first 40% saturated to concentrate the enterotoxin and then 15% saturated. The two precipitations were followed by gel filtration on Sephadex G-100. The enterotoxin appeared to be homogeneous on 7% polyacrylamide gel electrophoresis aft...
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ورودعنوان ژورنال:
- Applied microbiology
دوره 18 6 شماره
صفحات -
تاریخ انتشار 1969